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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated HGS Polyclonal antibody

HGS Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-10390

Print datasheet

Synonyms

HGS, HRS, Protein pp110, ZFYVE8

验证数据展示

  • IF Staining of HeLa using CL488-10390

    IF Staining of HeLa using CL488-10390

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 HGS antibody (CL488-10390) at dilution of 1:200.

经过测试的应用

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HGS Ready-To-Use IHC Kit. HGS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10390-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10390 targets HGS in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen HGS fusion protein Ag0589 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hepatocyte growth factor-regulated tyrosine kinase substrate
Synonyms HGS, HRS, Protein pp110, ZFYVE8
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC003565
Gene Symbol HGS
Gene ID (NCBI) 9146
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14964
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting the function of STAM which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein schwannomin/merlin. The growth suppression activity of schwannomin/merlin requires HGS and the binding of schwannomin/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HGS antibody CL488-10390Download protocol
Standard Protocols
Click here to view our Standard Protocols