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GRP78/BIP Monoclonal antibody, PBS Only

GRP78/BIP Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB,IP,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

1D6F7

Cat No : 66574-1-PBS

Print datasheet

Synonyms

BIP, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSPA5, MIF2

验证数据展示

  • WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

    WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • IP experiment of SGC-7901 using 66574-1-Ig (same clone as 66574-1-PBS)

    IP experiment of SGC-7901 using 66574-1-Ig (same clone as 66574-1-PBS)

    IP result of anti-GRP78/BIP (IP:66574-1-Ig, 5ug; Detection:66574-1-Ig 1:3000) with SGC-7901 cells lysate 2200 ug. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP78/HSPA5 antibody included
  • IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP78/HSPA5 antibody included
  • IF Staining of HeLa using 66574-1-Ig (same clone as 66574-1-PBS)

    IF Staining of HeLa using 66574-1-Ig (same clone as 66574-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 66574-1-Ig (same clone as 66574-1-PBS)

    FC experiment of MCF-7 using 66574-1-Ig (same clone as 66574-1-PBS)

    1X10^6 MCF-7 cells were stained with 0.2 ug Anti-Human GRP78/BIP (66574-1-Ig, Clone:1D6F7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy GRP78/HSPA5 Ready-To-Use IHC Kit. GRP78/HSPA5 primary antibody included.

产品信息

66574-1-PBS targets GRP78/BIP in WB, IP, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF,FC
Tested Reactivity Human, rat, mouse
Immunogen GRP78/BIP fusion protein Ag2188 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Synonyms BIP, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSPA5, MIF2
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 70-78 kDa
GenBank Accession NumberBC020235
Gene Symbol HSPA5
Gene ID (NCBI) 3309
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11021
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.