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  • Featured Product
  • KD/KO Validated

GNL3 Polyclonal antibody

GNL3 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 15060-1-AP

Print datasheet

Synonyms

C77032, E2 induced gene 3 protein, E2IG3, GNL3, NNP47, Novel nucleolar protein 47, NS, Nucleostemin

验证数据展示

  • WB analysis using 15060-1-AP

    WB analysis using 15060-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15060-1-AP (Nucleostemin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human oesophagus cancer using 15060-1-AP

    IHC staining of human oesophagus cancer using 15060-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human oesophagus cancer using 15060-1-AP

    IHC staining of human oesophagus cancer using 15060-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 15060-1-AP

    IF Staining of HeLa using 15060-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15060-1-AP (GNL3 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HeLa using 15060-1-AP

    IP experiment of HeLa using 15060-1-AP

    IP result of anti-GNL3 (IP:15060-1-AP, 4ug; Detection:15060-1-AP 1:600) with HeLa cells lysate 4000ug.

经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, HeLa cells, HepG2 cells, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman oesophagus cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15060-1-AP targets GNL3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen GNL3 fusion protein Ag7056 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanine nucleotide binding protein-like 3 (nucleolar)
Synonyms C77032, E2 induced gene 3 protein, E2IG3, GNL3, NNP47, Novel nucleolar protein 47, NS, Nucleostemin
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC001024
Gene Symbol GNL3
Gene ID (NCBI) 26354
RRIDAB_2263303
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BVP2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GNL3 antibody 15060-1-APDownload protocol
IHC protocol for GNL3 antibody 15060-1-APDownload protocol
IF protocol for GNL3 antibody 15060-1-APDownload protocol
IP protocol for GNL3 antibody 15060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Nanobiotechnology

Targeted micelles with chemotherapeutics and gene drugs to inhibit the G1/S and G2/M mitotic cycle of prostate cancer.

Authors - Yiran Zhang
View Article
humanWB

Cancer Manag Res

The oncogenic role of GNL3 in the progression and metastasis of osteosarcoma.

Authors - Tianyou Li
  • KD Validated
View Article