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CoraLite® Plus 488-conjugated GNAI2 Monoclonal antibody

GNAI2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3F6H5

Cat No : CL488-67007

Print datasheet

Synonyms

GIP, GNAI2, GNAI2B, H_LUCA15.1, H_LUCA16.1

验证数据展示

  • IF Staining of A431 using CL488-67007

    IF Staining of A431 using CL488-67007

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CL488-67007 (GNAI2 antibody) at dilution of 1:100.

经过测试的应用

Positive IF detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 67007-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67007 targets GNAI2 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen GNAI2 fusion protein Ag28560 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
Synonyms GIP, GNAI2, GNAI2B, H_LUCA15.1, H_LUCA16.1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC012138
Gene Symbol GNAI2
Gene ID (NCBI) 2771
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP04899
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GNAI2 antibody CL488-67007Download protocol
Standard Protocols
Click here to view our Standard Protocols