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CoraLite® Plus 488-conjugated GBP1 Monoclonal antibody

GBP1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A10E8

Cat No : CL488-67161

Print datasheet

Synonyms

GBP 1, GBP1, GTP binding protein 1, HuGBP 1

验证数据展示

  • IF Staining of MCF-7 using CL488-67161

    IF Staining of MCF-7 using CL488-67161

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 GBP1 antibody (CL488-67161, Clone: 2A10E8 ) at dilution of 1:100.

经过测试的应用

Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy GBP1 Ready-To-Use IHC Kit. GBP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67161-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67161 targets GBP1 in IF applications and shows reactivity with Human, pig, rat samples.

Tested Applications IF/ICC
Tested Reactivity Human, pig, rat
Immunogen GBP1 fusion protein Ag7767 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name GTP binding protein 1
Synonyms GBP 1, GBP1, GTP binding protein 1, HuGBP 1
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC002666
Gene Symbol GBP1
Gene ID (NCBI) 2633
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP32455
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by IFN and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP. GBP1 is induced in response to type I and type II IFNs and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GBP1 antibody CL488-67161Download protocol
Standard Protocols
Click here to view our Standard Protocols