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GAPDH Recombinant antibody

GAPDH Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H18

Cat No : 81640-5-RR

Print datasheet

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12

验证数据展示

  • WB analysis using 81640-5-RR

    WB analysis using 81640-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81640-5-RR (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 81640-5-RR

    IF Staining of HeLa using 81640-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GAPDH antibody (81640-5-RR, Clone: 1H18 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, NIH/3T3 cells, HSC-T6 cells, K-562 cells, Jurakat cells, HEK-293 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy GAPDH Ready-To-Use IHC Kit. GAPDH primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81640-5-RR targets GAPDH in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen GAPDH fusion protein Ag0766 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glyceraldehyde-3-phosphate dehydrogenase
Synonyms G3PD, GAPD, GAPDH, OK/SW cl.12
Calculated Molecular Weight 36 kDa
Observed Molecular Weight36 kDa
GenBank Accession NumberBC004109
Gene Symbol GAPDH
Gene ID (NCBI) 2597
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04406
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

实验方案

Product Specific Protocols
WB protocol for GAPDH antibody 81640-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols