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Fibrillin 1 Polyclonal antibody

Fibrillin 1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29425-1-AP

Print datasheet

Synonyms

FBN, FBN1, fibrillin 1, MASS, MFS1, OCTD, SGS, WMS

验证数据展示

  • WB analysis using 29425-1-AP

    WB analysis using 29425-1-AP

    HeLa cells were subjected to Tris-acetate gel system followed by western blot with 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 29425-1-AP

    IHC staining of human endometrial cancer using 29425-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 29425-1-AP

    IF Staining of A431 using 29425-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Fibrillin 1 antibody (29425-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IHC staining of human endometrial cancer using 29425-1-AP

    IHC staining of human endometrial cancer using 29425-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29425-1-AP targets Fibrillin 1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human
Immunogen Fibrillin 1 fusion protein Ag30283 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibrillin 1
Synonyms FBN, FBN1, fibrillin 1, MASS, MFS1, OCTD, SGS, WMS
Observed Molecular Weight350 kDa
GenBank Accession NumberBC146854
Gene Symbol FBN1
Gene ID (NCBI) 2200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35555
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibrillin 1 is a 350 kDa large extracellular matrix glycoprotein that formed thread-like microfibril filaments, which provided structural support for tissues, formed elastic fibers in the skin and vessels, regulated the availability of the transforming growth factor beta (TGF-β) protein, and modulated the microenvironment. Fibrillin 1 contains a large number of cysteine-rich epidermal growth factor (EGF)- like domains, most capable of binding calcium ions (cbEGF-like), and a few motifs homologous to the binding protein for transforming growth factor beta (TGFβ1-BP)- like module (PMID: 29220879, PMID: 35234370).

实验方案

Product Specific Protocols
WB protocol for Fibrillin 1 antibody 29425-1-APDownload protocol
IHC protocol for Fibrillin 1 antibody 29425-1-APDownload protocol
IF protocol for Fibrillin 1 antibody 29425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols