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CoraLite®594-conjugated Fetuin-A Monoclonal antibody

Fetuin-A Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F6B9

Cat No : CL594-66094

Print datasheet

Synonyms

A2HS, AHS, AHSG, alpha 2 HS glycoprotein, Alpha 2 Z globulin, Ba alpha 2 glycoprotein, FETUA, Fetuin A, Fetuin-A, HSGA

验证数据展示

  • IF Staining of HepG2 using CL594-66094

    IF Staining of HepG2 using CL594-66094

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66094 (Fetuin-A antibody) at dilution of 1:100.

  • FC experiment of HepG2 using CL594-66094

    FC experiment of HepG2 using CL594-66094

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Fetuin-A (CL594-66094, Clone:1F6B9) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy AHSG/Fetuin A Ready-To-Use IHC Kit. AHSG/Fetuin A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66094-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-66094 is Fetuin-A Fusion Protein expressed in E. coli.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human
Immunogen Fetuin-A fusion protein Ag10073 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name alpha-2-HS-glycoprotein
Synonyms A2HS, AHS, AHSG, alpha 2 HS glycoprotein, Alpha 2 Z globulin, Ba alpha 2 glycoprotein, FETUA, Fetuin A, Fetuin-A, HSGA
Calculated Molecular Weight 367 aa, 39 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC048198
Gene Symbol AHSG
Gene ID (NCBI) 197
RRIDAB_2883509
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02765
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fetuin-A (alpha-2-HS-glycoprotein, AHSG), a liver borne plasma protein, contributes to the prevention of soft tissue calcification, modulates inflammation, reduces INS sensitivity and fosters weight gain following high fat diet or ageing. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues.

实验方案

Product Specific Protocols
IF protocol for CL594 Fetuin-A antibody CL594-66094Download protocol
FC protocol for CL594 Fetuin-A antibody CL594-66094Download protocol
Standard Protocols
Click here to view our Standard Protocols