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FMR1 Polyclonal antibody

FMR1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

Cat No : 13755-1-AP

Print datasheet

Synonyms

FMR1, FMRP, fragile X mental retardation 1, FRAXA, POF, POF1, Protein FMR 1

验证数据展示

WB analysis of Jurkat cells using 13755-1-AP
WB result of FMR1 antibody (13755-1-AP, 1:1500) with si-Control and si-FMR1 transfected Jurkat cells. FMR1 gene has many isoforms with MW 59-72 kDa.
WB analysis of K-562 using 13755-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of Jurkat using 13755-1-AP
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FMR1 (13755-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IHC staining of human colon cancer using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP experiment of HeLa using 13755-1-AP
IP result of anti-FMR1 (IP:13755-1-AP, 4ug; Detection:13755-1-AP 1:1000) with HeLa cells lysate 2800ug.
IF Staining of HepG2 using 13755-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13755-1-AP (FMRP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 13755-1-AP

Size

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经过测试的应用

Positive WB detected in	K-562 cells, mouse brain tissue, mouse thymus tissue, Jurkat cells, HeLa cells, HEK-293 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon tissue, human liver tissue, human colon cancer tissue, human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells, Hela cells
Positive FC detected in	Jurkat cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)	IF : 1:50-1:500
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 6 publications below
IHC	See 2 publications below

产品信息

13755-1-AP targets FMR1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications	FC, IF/ICC, IHC, IP, WB, ELISA
Cited Applications	WB, IHC
Tested Reactivity	human, mouse
Cited Reactivity	human
Immunogen	FMR1 fusion protein Ag4697 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	fragile X mental retardation 1
Synonyms	FMR1, FMRP, fragile X mental retardation 1, FRAXA, POF, POF1, Protein FMR 1
Calculated Molecular Weight	632 aa, 71 kDa
Observed Molecular Weight	59-72 kDa
GenBank Accession Number	BC038998
Gene Symbol	FMR1
Gene ID (NCBI)	2332
RRID	AB_2262872
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q06787
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

实验方案

Product Specific Protocols
WB protocol for FMR1 antibody 13755-1-AP	Download protocol
IHC protocol for FMR1 antibody 13755-1-AP	Download protocol
IF protocol for FMR1 antibody 13755-1-AP	Download protocol
IP protocol for FMR1 antibody 13755-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human		Cell Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization. Authors - David W Sanders View Article
human	WB	Cancer Cell Int MiR-323a-3p acts as a tumor suppressor by suppressing FMR1 and predicts better esophageal squamous cell carcinoma outcome. Authors - Yu Men View Article
human	WB	Biology (Basel) In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome. Authors - Olga Gourdomichali View Article
human	WB,IHC	Cell Death Dis FMR1 promotes the progression of colorectal cancer cell by stabilizing EGFR mRNA in an m6A-dependent manner Authors - Yuhan Hu KD Validated View Article
human	WB	FASEB J Decreased DANCR contributes to high glucose-induced extracellular matrix accumulation in human renal mesangial cell via regulating the TGF-β/Smad signaling Authors - Feng Liu View Article
human	WB	Dev Cell Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome Authors - Ji Geng View Article

FMR1 Polyclonal antibody

FMR1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

验证数据展示

WB analysis of Jurkat cells using 13755-1-AP

WB analysis of K-562 using 13755-1-AP

IHC staining of human colon using 13755-1-AP

IHC staining of human colon using 13755-1-AP

FC experiment of Jurkat using 13755-1-AP

IHC staining of human colon cancer using 13755-1-AP

IHC staining of human colon cancer using 13755-1-AP

IP experiment of HeLa using 13755-1-AP

IF Staining of HepG2 using 13755-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.

MiR-323a-3p acts as a tumor suppressor by suppressing FMR1 and predicts better esophageal squamous cell carcinoma outcome.

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

FMR1 promotes the progression of colorectal cancer cell by stabilizing EGFR mRNA in an m6A-dependent manner

Decreased DANCR contributes to high glucose-induced extracellular matrix accumulation in human renal mesangial cell via regulating the TGF-β/Smad signaling

Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome

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Flow Cytometry Perm Buffer (10X)