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FMNL1 Polyclonal antibody

FMNL1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10395-1-AP

Print datasheet

Synonyms

C17orf1, C17orf1B, CLL associated antigen KW 13, FHOD4, FMNL, FMNL1, formin like 1, Formin like protein 1, KW 13, Leukocyte formin

验证数据展示

  • WB analysis of Raji using 10395-1-AP

    WB analysis of Raji using 10395-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10395-1-AP (FMNL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 10395-1-AP

    IHC staining of human small intestine using 10395-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10395-1-AP (FMNL1 antibody) at dilution of 1:200 (under 10x lens).

  • IF Staining of U2OS using 10395-1-AP

    IF Staining of U2OS using 10395-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using FMNL1 antibody (10395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of Saos-2 using 10395-1-AP

    IF Staining of Saos-2 using 10395-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using FMNL1 antibody (10395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 10395-1-AP

    WB analysis using 10395-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10395-1-AP (FMNL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRaji cells, Jurkat cells, HeLa cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, Saos-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:500
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10395-1-AP targets FMNL1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human
Immunogen FMNL1 fusion protein Ag0594 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name formin-like 1
Synonyms C17orf1, C17orf1B, CLL associated antigen KW 13, FHOD4, FMNL, FMNL1, formin like 1, Formin like protein 1, KW 13, Leukocyte formin
Calculated Molecular Weight 160 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC001710
Gene Symbol FMNL1
Gene ID (NCBI) 752
RRIDAB_10895969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95466
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. The MW of FMNL1 is about 150-160 kDa. (PMID: 16494944 )

实验方案

Product Specific Protocols
WB protocol for FMNL1 antibody 10395-1-APDownload protocol
IHC protocol for FMNL1 antibody 10395-1-APDownload protocol
IF protocol for FMNL1 antibody 10395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols