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FIS1 Recombinant antibody, PBS Only

FIS1 Recombinant Antibody for WB,IP,Indirect ELISA,IHC,IF,FC

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB,IP,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

6N15

Cat No : 82248-1-PBS

Print datasheet

Synonyms

CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11

验证数据展示

  • WB analysis using 82248-1-RR (same clone as 82248-1-PBS)

    WB analysis using 82248-1-RR (same clone as 82248-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82248-1-RR (FIS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 82248-1-RR (same clone as 82248-1-PBS)

    IP experiment of HeLa using 82248-1-RR (same clone as 82248-1-PBS)

    IP result of anti-FIS1 (IP:82248-1-RR, 4ug; Detection:82248-1-RR 1:5000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 82248-1-RR (same clone as 82248-1-PBS)

    IHC staining of human prostate cancer using 82248-1-RR (same clone as 82248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82248-1-RR (FIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82248-1-RR (same clone as 82248-1-PBS)

    IF Staining of HepG2 using 82248-1-RR (same clone as 82248-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FIS1 antibody (82248-1-RR, Clone: 6N15 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 82248-1-RR (same clone as 82248-1-PBS)

    FC experiment of HepG2 using 82248-1-RR (same clone as 82248-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FIS1 (82248-1-RR, Clone:6N15) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 82248-1-RR (same clone as 82248-1-PBS)

    IHC staining of rat brain using 82248-1-RR (same clone as 82248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82248-1-RR (FIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

产品信息

82248-1-PBS targets FIS1 in WB, IP, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF,FC
Tested Reactivity Human, mouse, rat
Immunogen FIS1 fusion protein Ag1409 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Synonyms CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11
Calculated Molecular Weight 17 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC009428
Gene Symbol FIS1
Gene ID (NCBI) 51024
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y3D6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (PMID: 21257639, 21459773, 23333625)