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- KD/KO Validated
FBXW7 Polyclonal antibody
FBXW7 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HEK-293 cells, MCF-7 cells, human brain tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human skin cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy FBXW7 Ready-To-Use IHC Kit. FBXW7 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 10 publications below |
产品信息
55290-1-AP targets FBXW7 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | F-box and WD repeat domain containing 7 |
| Synonyms | AGO, Archipelago homolog, CDC4, F box protein FBX30, FBW6, FBW7, FBX30, FBXO30, FBXW6, FBXW7, hAgo, hCdc4, SEL 10, SEL10 |
| Calculated Molecular Weight | 80 kDa |
| Observed Molecular Weight | 100-110 kDa, 66-75 kDa |
| GenBank Accession Number | NM_018315 |
| Gene Symbol | FBXW7 |
| Gene ID (NCBI) | 55294 |
| RRID | AB_2881300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969H0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FBXW7, also named as FBW7, FBX30, SEL10 and hAgo, is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It probably recognizes and binds to phosphorylated target proteins. FBXW7 is involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. FBXW7 is a general tumor suppressor in human cancer (PMID: 17909001). FBXW7 has 3 isoforms (α/β/γ) with the calculated molecular mass of 80 kDa, 70 kDa and 66 kDa, and apparent molecular mass of 100-110 kDa and 66-75 kDa (PMID: 18559665). This antibody is specific to FBXW7 and it can recognize all the isoforms of FBXW7.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXW7 antibody 55290-1-AP | Download protocol |
| IHC protocol for FBXW7 antibody 55290-1-AP | Download protocol |
| IF protocol for FBXW7 antibody 55290-1-AP | Download protocol |
| IP protocol for FBXW7 antibody 55290-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Cyclin E-induced replicative stress drives p53-dependent whole-genome duplication
| ||
iScience Polycomb repressive complex 2 binds and stabilizes NANOG to suppress differentiation-related genes to promote self-renewal | ||
Endocrinology Rictor regulates spermatogenesis by controlling Sertoli cell cytoskeletal organization and cell polarity in the mouse testis. | ||
Int J Biochem Cell Biol The deubiquitinase USP38 promotes cell proliferation through stabilizing c-Myc. | ||
