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CoraLite® Plus 647-conjugated FBL Monoclonal antibody

FBL Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3A9E8

Cat No : CL647-66985

Print datasheet

Synonyms

FBL, FIB, FIB1, fibrillarin, FLRN, RNU3IP1

验证数据展示

  • IF Staining of HepG2 using CL647-66985

    IF Staining of HepG2 using CL647-66985

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 FBL antibody (CL647-66985, Clone: 3A9E8 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL647-66985

    FC experiment of HepG2 using CL647-66985

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human FBL (CL647-66985, Clone:3A9E8)(red), or 0.8 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy FBL Ready-To-Use IHC Kit. FBL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66985-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66985 targets FBL in IF, FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, rat, mouse
Immunogen FBL fusion protein Ag8841 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fibrillarin
Synonyms FBL, FIB, FIB1, fibrillarin, FLRN, RNU3IP1
Calculated Molecular Weight 321 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC019260
Gene Symbol FBL
Gene ID (NCBI) 2091
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22087
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 FBL antibody CL647-66985Download protocol
FC protocol for CL Plus 647 FBL antibody CL647-66985Download protocol
Standard Protocols
Click here to view our Standard Protocols