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CoraLite® Plus 488-conjugated ELAVL4 Monoclonal antibody

ELAVL4 Monoclonal Antibody for FC (Intra), IF/ICC,IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse, rabbit, pig

Applications

FC (Intra), IF/ICC,IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B1F5

Cat No : CL488-67835

Print datasheet

Synonyms

ELAV like protein 4, ELAVL4, Hu antigen D, HUD, PNEM

验证数据展示

  • FC experiment of U2OS using CL488-67835

    FC experiment of U2OS using CL488-67835

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ELAVL4 (CL488-67835, Clone:1B1F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse brain using CL488-67835

    IF Staining of mouse brain using CL488-67835

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 ELAVL4 antibody (CL488-67835, Clone: 1B1F5 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inmouse brain tissue, U2OS cells
Positive FC detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 67835-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67835 targets ELAVL4 in IF, FC (Intra) applications and shows reactivity with Human, rat, mouse, rabbit, pig samples.

Tested Applications FC (Intra), IF/ICC,IF-P
Tested Reactivity Human, rat, mouse, rabbit, pig
Immunogen ELAVL4 fusion protein Ag3678 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
Synonyms ELAV like protein 4, ELAVL4, Hu antigen D, HUD, PNEM
Calculated Molecular Weight 380 aa, 42 kDa
Observed Molecular Weight32-40 kDa
GenBank Accession NumberBC036071
Gene Symbol ELAVL4
Gene ID (NCBI) 1996
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26378
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELAVL4, also named as HuD, is a 380 amino acid protein. The full name is neuronal embryonic lethal altered visual system-like(ELAVL) RNA-binding protein. ELAVL4 is expressed in brain and localized in the nucleus. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ELAVL4 antibody CL488-67835Download protocol
FC protocol for CL Plus 488 ELAVL4 antibody CL488-67835Download protocol
Standard Protocols
Click here to view our Standard Protocols