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CoraLite®594-conjugated EEA1 Monoclonal antibody

EEA1 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E11E4

Cat No : CL594-68065

Print datasheet

Synonyms

early endosome antigen 1, EEA1, MST105, MSTP105, ZFYVE2

验证数据展示

  • IF Staining of HepG2 using CL594-68065

    IF Staining of HepG2 using CL594-68065

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 EEA1 antibody (CL594-68065, Clone: 1E11E4 ) at dilution of 1:200, CL488-Phalloidin (green).

  • FC experiment of HeLa using CL594-68065

    FC experiment of HeLa using CL594-68065

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human EEA1 (CL594-68065, Clone:1E11E4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68065-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-68065 targets EEA1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, Mouse, Rat
Immunogen EEA1 fusion protein Ag28814 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name early endosome antigen 1
Synonyms early endosome antigen 1, EEA1, MST105, MSTP105, ZFYVE2
Calculated Molecular Weight 162 kDa
Observed Molecular Weight 162 kDa
GenBank Accession NumberNM_003566
Gene Symbol EEA1
Gene ID (NCBI) 8411
RRIDAB_2920189
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15075
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.

实验方案

Product Specific Protocols
IF protocol for CL594 EEA1 antibody CL594-68065Download protocol
FC protocol for CL594 EEA1 antibody CL594-68065Download protocol
Standard Protocols
Click here to view our Standard Protocols