EEA1 Recombinant antibody
EEA1 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2C11
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, LNCaP cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
81245-1-RR targets EEA1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | WB, ELISA |
| Tested Reactivity | Human, Mouse, Rat |
| Immunogen | EEA1 fusion protein Ag28814 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | early endosome antigen 1 |
| Synonyms | early endosome antigen 1, EEA1, MST105, MSTP105, ZFYVE2 |
| Calculated Molecular Weight | 162 kDa |
| Observed Molecular Weight | 162 kDa |
| GenBank Accession Number | NM_003566 |
| Gene Symbol | EEA1 |
| Gene ID (NCBI) | 8411 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15075 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EEA1 antibody 81245-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |