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EEA1 Monoclonal antibody, PBS Only

EEA1 Monoclonal Antibody for WB,IF,Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,IF,Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E11E4

Cat No : 68065-1-PBS

Print datasheet

Synonyms

early endosome antigen 1, EEA1, MST105, MSTP105, ZFYVE2

验证数据展示

  • IF Staining of HepG2 using 68065-1-Ig (same clone as 68065-1-PBS)

    IF Staining of HepG2 using 68065-1-Ig (same clone as 68065-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EEA1 antibody (68065-1-Ig, Clone: 1E11E4 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

  • WB analysis using 68065-1-Ig (same clone as 68065-1-PBS)

    WB analysis using 68065-1-Ig (same clone as 68065-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68065-1-Ig (EEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

产品信息

68065-1-PBS targets EEA1 in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,IF,Indirect ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen EEA1 fusion protein Ag28814 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name early endosome antigen 1
Synonyms early endosome antigen 1, EEA1, MST105, MSTP105, ZFYVE2
Calculated Molecular Weight 162 kDa
Observed Molecular Weight 162 kDa
GenBank Accession NumberNM_003566
Gene Symbol EEA1
Gene ID (NCBI) 8411
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15075
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.