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  • Featured Product
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CoraLite® Plus 488-conjugated Desmoplakin Polyclonal antibody

Desmoplakin Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-25318

Print datasheet

Synonyms

desmoplakin, DP, DPI, DPII, DSP

验证数据展示

  • IF Staining of A431 using CL488-25318

    IF Staining of A431 using CL488-25318

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 Desmoplakin antibody (CL488-25318) at dilution of 1:200.

  • FC experiment of A431 using CL488-25318

    FC experiment of A431 using CL488-25318

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Desmoplakin (CL488-25318) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inA431 cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 25318-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25318 targets Desmoplakin in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Immunogen Desmoplakin fusion protein Ag18065 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name desmoplakin
Synonyms desmoplakin, DP, DPI, DPII, DSP
Calculated Molecular Weight 2871 aa, 332 kDa
Observed Molecular Weight 240-330 kDa
GenBank Accession NumberBC140802
Gene Symbol DSP
Gene ID (NCBI) 1832
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15924
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Desmoplakin antibody CL488-25318Download protocol
FC protocol for CL Plus 488 Desmoplakin antibody CL488-25318Download protocol
Standard Protocols
Click here to view our Standard Protocols