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TID1 Polyclonal antibody

TID1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11088-1-AP

Print datasheet

Synonyms

DnaJ protein Tid 1, DNAJA3, HCA57, hTid 1, TID 1, TID1

验证数据展示

  • WB analysis of Jurkat using 11088-1-AP

    WB analysis of Jurkat using 11088-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11088-1-AP (TID1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 11088-1-AP

    IHC staining of human lung cancer using 11088-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11088-1-AP (TID1 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of K-562 using 11088-1-AP

    IP experiment of K-562 using 11088-1-AP

    IP result of anti-TID1 (IP:11088-1-AP, 4ug; Detection:11088-1-AP 1:500) with K-562 cells lysate 3200ug.

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11088-1-AP targets TID1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TID1 fusion protein Ag1558 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily A, member 3
Synonyms DnaJ protein Tid 1, DNAJA3, HCA57, hTid 1, TID 1, TID1
Calculated Molecular Weight 480 aa, 53 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC011855
Gene Symbol DNAJA3
Gene ID (NCBI) 9093
RRIDAB_2293102
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EY1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TID1 antibody 11088-1-APDownload protocol
IHC protocol for TID1 antibody 11088-1-APDownload protocol
IP protocol for TID1 antibody 11088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

Role of human HSPE1 for OPA1 processing independent of HSPD1

Authors - Nelson Yeung
View Article