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CoraLite® Plus 555-conjugated Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat No : CL555-10712

Print datasheet

Synonyms

CK 19, CK19, Cytokeratin 19, K19, K1CS, keratin 19, KRT19

验证数据展示

  • IF Staining of HeLa using CL555-10712

    IF Staining of HeLa using CL555-10712

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 555 Cytokeratin 19 antibody (CL555-10712) at dilution of 1:200.

  • FC experiment of A431 using CL555-10712

    FC experiment of A431 using CL555-10712

    1X10^6 A431 cells were intracellularly stained with 0.2 ug CoraLite® Plus 555 Anti-Human Cytokeratin 19 (CL555-10712) (red), or 0.2 ug CL555 Rabbit Isotype Control Antibody (CL555-30000)(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10712-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-10712 targets Cytokeratin 19 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse
Immunogen Cytokeratin 19 fusion protein Ag1085 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 19
Synonyms CK 19, CK19, Cytokeratin 19, K19, K1CS, keratin 19, KRT19
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 44-50 kDa
GenBank Accession NumberBC007628
Gene Symbol KRT19
Gene ID (NCBI) 3880
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08727
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

实验方案

Product Specific Protocols
IF protocol for CL Plus 555 Cytokeratin 19 antibody CL555-10712Download protocol
FC protocol for CL Plus 555 Cytokeratin 19 antibody CL555-10712Download protocol
Standard Protocols
Click here to view our Standard Protocols