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CoraLite®594-conjugated Cyclin H Monoclonal antibody

Cyclin H Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2C5G11

Cat No : CL594-67065

Print datasheet

Synonyms

CAK, CCNH, cyclin H, MO15 associated protein, p34, p37

验证数据展示

  • IF Staining of PC-3 using CL594-67065

    IF Staining of PC-3 using CL594-67065

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using CoraLite®594 Cyclin H antibody (CL594-67065, Clone: 2C5G11 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of PC-3 using CL594-67065

    FC experiment of PC-3 using CL594-67065

    1X10^6 PC-3 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Cyclin H (CL594-67065, Clone:2C5G11) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inPC-3 cells
Positive FC detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 67065-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67065 targets Cyclin H in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen Cyclin H fusion protein Ag28556 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name cyclin H
Synonyms CAK, CCNH, cyclin H, MO15 associated protein, p34, p37
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC005280
Gene Symbol CCNH
Gene ID (NCBI) 902
RRIDAB_2920082
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51946
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 Cyclin H antibody CL594-67065Download protocol
FC protocol for CL594 Cyclin H antibody CL594-67065Download protocol
Standard Protocols
Click here to view our Standard Protocols