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ChromoTek CoraLite® Plus 555-conjugated TIGIT VHH

CL555-tgt targets TIGIT in FC and IF applications and shows reactivity with Human samples.

Specificity/Target

TIGIT-Tag

Applications

FC, IF

Type

Nanobody

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat No : CL555-tgt

Synonyms

TIGIT-Nanobody, TIGIT-single domain antibody (sdAb), DKFZp667A205; FLJ39873; VSIG9; VSTM3

Validation Data Gallery

  • 1X10^6 CHO TIGIT stable expressing cells (red) and CHO parental cells were surface stained with 0.25 µg CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt). Cells were not fixed.

    1X10^6 CHO TIGIT stable expressing cells (red) and CHO parental cells were surface stained with 0.25 µg CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt). Cells were not fixed.

  • 1x10^6 PBMC were stained with 1ug CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt) and 5ul CoraLite® Plus 405 conjugated Anti-Human CD3 (Clone:UCHT1; CL405-65151). Lymphocytes were gated.

    1x10^6 PBMC were stained with 1ug CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt) and 5ul CoraLite® Plus 405 conjugated Anti-Human CD3 (Clone:UCHT1; CL405-65151). Lymphocytes were gated.

  • Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, yellow). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired with 20x objective and post-processed.  Note: Immunostaining with CL555-tgt can also be performed after formaldehyde fixation of the cells.

    Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, yellow). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired with 20x objective and post-processed.  Note: Immunostaining with CL555-tgt can also be performed after formaldehyde fixation of the cells.

  • Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, orange). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired and post-processed with Leica Thunder Imager, 40x objective.  Note: Immunostaining with CL555-tgt can also be performed after formaldehyde fixation of the cells.

    Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, orange). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired and post-processed with Leica Thunder Imager, 40x objective.  Note: Immunostaining with CL555-tgt can also be performed after formaldehyde fixation of the cells.

  • Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta). After formaldehyde fixation, triton permeabilization and blocking with BSA, the intracellular pool of TIGIT was immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, yellow). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired and post-processed with Leica Thunder Imager, 40x objective.

    Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta). After formaldehyde fixation, triton permeabilization and blocking with BSA, the intracellular pool of TIGIT was immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, yellow). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired and post-processed with Leica Thunder Imager, 40x objective.

  • Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, yellow). After formaldehyde fixation, triton permeabilization and blocking with BSA, the intracellular pool of TIGIT was immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.

    Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, yellow). After formaldehyde fixation, triton permeabilization and blocking with BSA, the intracellular pool of TIGIT was immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.

Product Information

CL555-tgt targets TIGIT in FC and IF applications and shows reactivity with Human samples.

ApplicationsFC, IF
ReactivityHuman
ConjugateCoraLite® Plus 555 Fluorescent Dye
Type Nanobody
ClassRecombinant
HostAlpaca
AffinityPicomolar range, below the assay limit (biolayer interferometry)
Molecular Weight14.346 kDa
FormLiquid
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
RRID/
Storage Buffer500 mM NaCl, 10 mM HEPES pH 7.0, 5 mM EDTA
Storage ConditionStore at -20°C; Shipped at ambient temperature.
BackgroundTIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).