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Cardiac Troponin I Monoclonal antibody, PBS Only

Cardiac Troponin I Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

1D5D6

Cat No : 66376-1-PBS

Print datasheet

Synonyms

Cardiac troponin I, CMD2A, CMH7, cTnI, RCM1, TNNC1, TNNI3, troponin I type 3 (cardiac), Troponin I, cardiac muscle

验证数据展示

  • WB analysis using 66376-1-Ig (same clone as 66376-1-PBS)

    WB analysis using 66376-1-Ig (same clone as 66376-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin I antibody (66376-1-Ig, Clone: 1D5D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    IHC staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • WB analysis of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    WB analysis of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Human heart tissue and skeletal muscle tissue (negative control) were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I Antibody) at dilution of 1:20,000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    WB analysis of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Mouse heart tissue (6 ug/lane) was subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I Antibody) at dilution of 1:5,000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed mouse heart tissue using 66376-1-Ig(Cardiac Troponin I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

产品信息

66376-1-PBS targets Cardiac Troponin I in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,IHC,IF,ELISA
Tested Reactivity human, mouse, rat
Immunogen Cardiac Troponin I fusion protein Ag16758 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name troponin I type 3 (cardiac)
Synonyms Cardiac troponin I, CMD2A, CMH7, cTnI, RCM1, TNNC1, TNNI3, troponin I type 3 (cardiac), Troponin I, cardiac muscle
Calculated Molecular Weight 210 aa, 24 kDa
Observed Molecular Weight 26-28 kDa
GenBank Accession NumberBC096165
Gene Symbol TNNI3
Gene ID (NCBI) 7137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19429
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI.