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Calsequestrin 2 Monoclonal antibody, PBS Only

Calsequestrin 2 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, pig, mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1C10A1

Cat No : 66419-1-PBS

Print datasheet

Synonyms

Calsequestrin 2, CASQ2, PDIB2

验证数据展示

  • WB analysis using 66419-1-Ig (same clone as 66419-1-PBS)

    WB analysis using 66419-1-Ig (same clone as 66419-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66419-1-Ig (same clone as 66419-1-PBS)

    IHC staining of human heart using 66419-1-Ig (same clone as 66419-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66419-1-Ig (same clone as 66419-1-PBS)

    IHC staining of human heart using 66419-1-Ig (same clone as 66419-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 66419-1-Ig (same clone as 66419-1-PBS)

    IF Staining of mouse heart using 66419-1-Ig (same clone as 66419-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calsequestrin 2 antibody (66419-1-Ig, Clone: 1C10A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 66419-1-Ig (same clone as 66419-1-PBS)

    IF Staining of mouse heart using 66419-1-Ig (same clone as 66419-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calsequestrin 2 antibody (66419-1-Ig, Clone: 1C10A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

产品信息

66419-1-PBS targets Calsequestrin 2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF
Tested Reactivity human, rat, pig, mouse
Immunogen Calsequestrin 2 fusion protein Ag13246 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name calsequestrin 2 (cardiac muscle)
Synonyms Calsequestrin 2, CASQ2, PDIB2
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC022288
Gene Symbol CASQ2
Gene ID (NCBI) 845
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14958
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.