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Calretinin Monoclonal antibody, PBS Only

Calretinin Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2D7A9

Cat No : 66496-1-PBS

Print datasheet

Synonyms

29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR

验证数据展示

  • WB analysis using 66496-1-Ig (same clone as 66496-1-PBS)

    WB analysis using 66496-1-Ig (same clone as 66496-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
  • IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
  • IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
  • IF Staining of SH-SY5Y using 66496-1-Ig (same clone as 66496-1-PBS)

    IF Staining of SH-SY5Y using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
Planning an IHC experiment? We recommend our IHCeasy Calretinin Ready-To-Use IHC Kit. Calretinin primary antibody included.

产品信息

66496-1-PBS targets Calretinin in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF
Tested Reactivity Human, mouse, pig, rat
Immunogen Calretinin fusion protein Ag2924 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calbindin 2
Synonyms 29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC015484
Gene Symbol CALB2
Gene ID (NCBI) 794
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22676
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.