CRP Monoclonal antibody
CRP Monoclonal Antibody for IF/ICC, IHC, WB,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig and More (1)
Applications
IF/ICC, IHC, WB,ELISA
Conjugate
Unconjugated
CloneNo.
3F10A11
验证数据展示
经过测试的应用
Positive WB detected in | serum from mouse injected with bacteria, pig liver tissue, rat liver tissue, serum from mouse injected with bacteria tissue |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human liver tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66250-1-Ig targets CRP in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | IF/ICC, IHC, WB,ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat, monkey |
Immunogen | CRP fusion protein Ag9883 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | C-reactive protein, pentraxin-related |
Synonyms | C reactive protein, CRP, PTX1 |
Calculated Molecular Weight | 224 aa, 25 kDa |
Observed Molecular Weight | 27 kDa |
GenBank Accession Number | BC020766 |
Gene Symbol | CRP |
Gene ID (NCBI) | 1401 |
RRID | AB_2881638 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P02741 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CRP antibody 66250-1-Ig | Download protocol |
IHC protocol for CRP antibody 66250-1-Ig | Download protocol |
IF protocol for CRP antibody 66250-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
Front Immunol Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline Structures. | ||
Sci Rep A label-free fiber optic SPR biosensor for specific detection of C-reactive protein. | ||
Sci Rep C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1. | ||
Brain Behav Prenatal stress induced depressive-like behavior and region dependently high CRP level in offspring rats. | ||
Int J Radiat Oncol Biol Phys Identification of Novel Biomarkers for Acute Radiation Injury Using Multiomics Approach and Nonhuman Primate Model |