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CRM1 Monoclonal antibody, PBS Only

CRM1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2D3B6

Cat No : 66763-1-PBS

Print datasheet

Synonyms

CRM1, DKFZp686B1823, Exp1, Exportin 1, XPO1

验证数据展示

  • WB analysis using 66763-1-Ig (same clone as 66763-1-PBS)

    WB analysis using 66763-1-Ig (same clone as 66763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66763-1-Ig (CRM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66763-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66763-1-Ig (same clone as 66763-1-PBS)

    IHC staining of human breast cancer using 66763-1-Ig (same clone as 66763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66763-1-Ig (CRM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66763-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CRM1 antibody included
  • IHC staining of human breast cancer using 66763-1-Ig (same clone as 66763-1-PBS)

    IHC staining of human breast cancer using 66763-1-Ig (same clone as 66763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66763-1-Ig (CRM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66763-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CRM1 antibody included
  • IF Staining of HeLa using 66763-1-Ig (same clone as 66763-1-PBS)

    IF Staining of HeLa using 66763-1-Ig (same clone as 66763-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CRM1 antibody (66763-1-Ig, Clone: 2D3B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66763-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CRM1 Ready-To-Use IHC Kit. CRM1 primary antibody included.

产品信息

66763-1-PBS targets CRM1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF
Tested Reactivity Human, Mouse, Rat
Immunogen CRM1 fusion protein Ag27337 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name exportin 1 (CRM1 homolog, yeast)
Synonyms CRM1, DKFZp686B1823, Exp1, Exportin 1, XPO1
Calculated Molecular Weight 1071 aa, 123 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC032847
Gene Symbol XPO1
Gene ID (NCBI) 7514
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14980
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).