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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated COMMD1 Polyclonal antibody

COMMD1 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-11938

Print datasheet

Synonyms

C2orf5, COMMD1, MURR1, Protein Murr1

验证数据展示

  • FC experiment of HepG2 using CL488-11938

    FC experiment of HepG2 using CL488-11938

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human COMMD1 (CL488-11938) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-11938

    IF Staining of HepG2 using CL488-11938

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 COMMD1 antibody (CL488-11938) at dilution of 1:200.

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy COMMD1 Ready-To-Use IHC Kit. COMMD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11938-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11938 targets COMMD1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Immunogen COMMD1 fusion protein Ag2535 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name copper metabolism (Murr1) domain containing 1
Synonyms C2orf5, COMMD1, MURR1, Protein Murr1
Calculated Molecular Weight 190 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC022046
Gene Symbol COMMD1
Gene ID (NCBI) 150684
RRIDAB_2919045
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen Affinity Purified
UNIPROT IDQ8N668
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 COMMD1 antibody CL488-11938Download protocol
FC protocol for CL Plus 488 COMMD1 antibody CL488-11938Download protocol
Standard Protocols
Click here to view our Standard Protocols