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CLN5 Polyclonal antibody

CLN5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 24605-1-AP

Print datasheet

Synonyms

CLN5, NCL, Protein CLN5

验证数据展示

  • WB analysis using 24605-1-AP

    WB analysis using 24605-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24605-1-AP (CLN5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24605-1-AP targets CLN5 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA
Tested Reactivity human, mouse
Immunogen CLN5 fusion protein Ag18175 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ceroid-lipofuscinosis, neuronal 5
Synonyms CLN5, NCL, Protein CLN5
Calculated Molecular Weight 407 aa, 46 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC153154
Gene Symbol CLN5
Gene ID (NCBI) 1203
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75503
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The CLN5 (ceroid lipofuscinosis neuronal protein 5) protein is ubiquitously expressed in the majority of tissues studied and in the brain, CLN5 shows both neuronal and glial cell expression. CLN5 plays a role in maintaining an acidic environment in the lysosomes, a critical feature for a functional lysosome (PMID: 38236309). Moreover, CLN5-deficient neuronal progenitor cells showed reduced thioesterase activity, confirming the live cell function of CLN5 in setting S-depalmitoylation levels (PMID: 35427157).

实验方案

Product Specific Protocols
WB protocol for CLN5 antibody 24605-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols