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CEACAM7 Polyclonal antibody

CEACAM7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24626-1-AP

Print datasheet

Synonyms

Carcinoembryonic antigen CGM2, CEA, CEACAM7, CGM2

验证数据展示

  • WB analysis of mouse colon using 24626-1-AP

    WB analysis of mouse colon using 24626-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 24626-1-AP (CEACAM7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 24626-1-AP

    IHC staining of human colon using 24626-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24626-1-AP (CEACAM7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse colon tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24626-1-AP targets CEACAM7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen CEACAM7 fusion protein Ag18579 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carcinoembryonic antigen-related cell adhesion molecule 7
Synonyms Carcinoembryonic antigen CGM2, CEA, CEACAM7, CGM2
Calculated Molecular Weight 265 aa, 29 kDa
Observed Molecular Weight58 kDa
GenBank Accession NumberBC121132
Gene Symbol CEACAM7
Gene ID (NCBI) 1087
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14002
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEACAM7, also known as CGM2, belongs to the carcinoembryonic antigen (CEA) family. CEACAM7 contains three domains: an N-terminal IgV domain, a single IgC2 domain, and a cell-surface GPI anchor domain. CEACAM7 achieves cell adhesion through dimerization of the N-terminal IgV domain. CEACAM7 is expressed on highly differentiated epithelial cells of the colon and rectum and on the epithelial cells within the ducts of the pancreas (PMID: 26323304). CEACAM7 has 2 isoforms with the molecular mass of 19 and 29 kDa. The observed MW of CEACAM7 is 58 kDa, which is consistent with the description in the literature (PMID: 36828119).

实验方案

Product Specific Protocols
WB protocol for CEACAM7 antibody 24626-1-APDownload protocol
IHC protocol for CEACAM7 antibody 24626-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols