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CD97 Monoclonal antibody

CD97 Monoclonal Antibody for FC, IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

FC, IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3F10C2

Cat No : 66972-1-Ig

Print datasheet

Synonyms

CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, TM7LN1

验证数据展示

  • IF Staining of Jurkat using 66972-1-Ig

    IF Staining of Jurkat using 66972-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD97 antibody (66972-1-Ig, Clone: 3F10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of K-562 using 66972-1-Ig

    WB analysis of K-562 using 66972-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 66972-1-Ig

    FC experiment of Jurkat using 66972-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human CD97 (66972-1-Ig, Clone:3F10C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inK-562 cells, HepG2 cells, HL-60 cells, DC2.4 cells
Positive IF detected inJurkat cells
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66972-1-Ig targets CD97 in WB, IF, FC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications FC, IF/ICC, WB, ELISA
Tested Reactivity Human, Mouse
Immunogen CD97 fusion protein Ag27991 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CD97 molecule
Synonyms CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, TM7LN1
Calculated Molecular Weight 722 aa, 79 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC026690
Gene Symbol CD97
Gene ID (NCBI) 976
RRIDAB_2882293
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48960
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).

实验方案

Product Specific Protocols
WB protocol for CD97 antibody 66972-1-IgDownload protocol
IF protocol for CD97 antibody 66972-1-IgDownload protocol
FC protocol for CD97 antibody 66972-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols