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CD9 Recombinant antibody, PBS Only

CD9 Recombinant Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

5K14

Cat No : 82105-1-PBS

Print datasheet

Synonyms

5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, DRAP 27, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29

验证数据展示

  • WB analysis using 82105-1-RR (same clone as 82105-1-PBS)

    WB analysis using 82105-1-RR (same clone as 82105-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82105-1-RR (CD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 82105-1-RR (same clone as 82105-1-PBS)

    IHC staining of human lung cancer using 82105-1-RR (same clone as 82105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IHC staining of human lung cancer using 82105-1-RR (same clone as 82105-1-PBS)

    IHC staining of human lung cancer using 82105-1-RR (same clone as 82105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IF Staining of MCF-7 using 82105-1-RR (same clone as 82105-1-PBS)

    IF Staining of MCF-7 using 82105-1-RR (same clone as 82105-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (82105-1-RR, Clone: 5K14 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.

产品信息

82105-1-PBS targets CD9 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IHC,IF
Tested Reactivity Human
Immunogen CD9 fusion protein Ag14546 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD9 molecule
Synonyms 5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, DRAP 27, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29
Calculated Molecular Weight 228 aa, 25 kDa
Observed Molecular Weight23-27 kDa
GenBank Accession NumberBC011988
Gene Symbol CD9
Gene ID (NCBI) 928
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21926
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and support myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in fertilization of mammals (PMID: 25536312).