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Anti-Human PD-1/CD279 (EH12.2H7)

PD-1/CD279 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Non-human primates, Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

EH12.2H7

Cat No : 65168-1-Ig

Print datasheet

Synonyms

CD279, hPD 1, hPD l, PD1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

验证数据展示

  • FC experiment of human PBMCs using 65168-1-Ig

    FC experiment of human PBMCs using 65168-1-Ig

    1X10^6 day 3 PHA treated human PBMCs were surface stained with 0.2 ug Anti-Human PD-1/CD279 (65168-1-Ig, Clone:EH12.2H7) and CL594-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. Blue line indicates unstained stimulated cells. Black dashed line indicates unstimulated stained cells.

经过测试的应用

Positive FC detected inPHA treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65168-1-Ig targets PD-1/CD279 in FC applications and shows reactivity with Non-human primates, Human samples.

Tested Applications FC
Tested Reactivity Non-human primates, Human
Immunogen Recombinant human CD279 protein 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name programmed cell death 1
Synonyms CD279, hPD 1, hPD l, PD1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2
Calculated Molecular Weight 288 aa, 32 kDa
GenBank Accession NumberBC074740
Gene Symbol PDCD1
Gene ID (NCBI) 5133
RRIDAB_2918445
Conjugate Unconjugated
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDQ15116
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

实验方案

Product Specific Protocols
FC protocol for PD-1/CD279 antibody 65168-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols