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CD20 Monoclonal antibody, PBS Only

CD20 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

4A7G3

Cat No : 60271-1-PBS

Print datasheet

Synonyms

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

验证数据展示

  • WB analysis using 60271-1-Ig (same clone as 60271-1-PBS)

    WB analysis using 60271-1-Ig (same clone as 60271-1-PBS)

    Raji cells (CD20+), Daudi cells (CD20+), Ramos cells (CD20+), Jurkat cells (CD20-), and HeLa cells (CD20-) were subjected to SDS PAGE followed by western blot with 60271-1-Ig (CD20 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD20 antibody included
  • IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD20 antibody included
  • IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD3 antibody (17617-1-AP, green). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD3 antibody (17617-1-AP, green). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • FC experiment of Daudi using 60271-1-Ig (same clone as 60271-1-PBS)

    FC experiment of Daudi using 60271-1-Ig (same clone as 60271-1-PBS)

    1X10^6 Daudi cells were intracellularly stained with 0.4 ug Anti-Human CD20 (60271-1-Ig, Clone:4A7G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.

产品信息

60271-1-PBS targets CD20 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,Indirect ELISA,IHC,IF,FC
Tested Reactivity human
Immunogen CD20 fusion protein Ag17309 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name membrane-spanning 4-domains, subfamily A, member 1
Synonyms B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7
Calculated Molecular Weight 297 aa, 33 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC002807
Gene Symbol MS4A1
Gene ID (NCBI) 931
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11836
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.