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  • Featured Product
  • KD/KO Validated

CD107b / LAMP2 Monoclonal antibody, PBS Only

CD107b / LAMP2 Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgA

Reactivity

human, mouse, pig

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

2D3B9

Cat No : 66301-1-PBS

Print datasheet

Synonyms

CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker

验证数据展示

  • WB analysis using 66301-1-Ig (same clone as 66301-1-PBS)

    WB analysis using 66301-1-Ig (same clone as 66301-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66301-1-Ig (same clone as 66301-1-PBS)

    IF Staining of HeLa using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66301-1-Ig (LAMP2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), phalloidine stains F-actin (red). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66301-1-Ig (same clone as 66301-1-PBS)

    IHC staining of human liver cancer using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LAMP2 antibody included
  • IHC staining of human liver cancer using 66301-1-Ig (same clone as 66301-1-PBS)

    IHC staining of human liver cancer using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LAMP2 antibody included
Planning an IHC experiment? We recommend our IHCeasy LAMP2 Ready-To-Use IHC Kit. LAMP2 primary antibody included.

产品信息

66301-1-PBS targets CD107b / LAMP2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Applications WB,IHC,IF,ELISA
Tested Reactivity human, mouse, pig
Immunogen CD107b / LAMP2 fusion protein Ag0595 种属同源性预测
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Full Name lysosomal-associated membrane protein 2
Synonyms CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC002965
Gene Symbol LAMP2
Gene ID (NCBI) 3920
ENSEMBL Gene IDENSG00000005893
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13473
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides.