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Caspase 12 Polyclonal antibody

Caspase 12 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 55238-1-AP

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Synonyms

CASP 12, CASP12, CASP12P1, Caspase 12, caspase 12 (gene/pseudogene), Caspase12, Inactive caspase 12



经过测试的应用

Positive WB detected inHEK-293 cells, multi-cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate hyperplasia tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55238-1-AP targets Caspase 12 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman, hamster, pig
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caspase 12 (gene/pseudogene)
Synonyms CASP 12, CASP12, CASP12P1, Caspase 12, caspase 12 (gene/pseudogene), Caspase12, Inactive caspase 12
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 36-42 kDa, 50 kDa
GenBank Accession NumberNR_000035
Gene Symbol CASP12
Gene ID (NCBI) 120329
RRIDAB_10892670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. It is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels. Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (50 kDa) and its cleaved product (40-44 kDa). This antibody does not cross-react with other caspases.

实验方案

Product Specific Protocols
WB protocol for Caspase 12 antibody 55238-1-APDownload protocol
IHC protocol for Caspase 12 antibody 55238-1-APDownload protocol
IF protocol for Caspase 12 antibody 55238-1-APDownload protocol
IP protocol for Caspase 12 antibody 55238-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Autophagy

SARS-CoV-2 ORF3a induces RETREG1/FAM134B-dependent reticulophagy and triggers sequential ER stress and inflammatory responses during SARS-CoV-2 infection.

Authors - Xiaolin Zhang
humanWB

ACS Appl Mater Interfaces

Endoplasmic Reticulum-Targeted Aggregation-Induced Emission Luminogen for Synergetic Tumor Ablation with Glibenclamide

Authors - Yifan Wu
WB

J Hazard Mater

Perinatal low-dose PBDE-47 exposure hampered thyroglobulin turnover and induced thyroid cell apoptosis by triggering ER stress and lysosomal destabilization contributing to thyroid toxicity in adult female rats.

Authors - Pei Li
humanWB,IF

Theranostics

Exosomes from mesenchymal stem cells modulate endoplasmic reticulum stress to protect against nucleus pulposus cell death and ameliorate intervertebral disc degeneration in vivo.

Authors - Zhiwei Liao
humanWB

Biomed Pharmacother

A novel synthetic curcumin derivative MHMM-41 induces ROS-mediated apoptosis and migration blocking of human lung cancer cells A549.

Authors - Guang-Zhou Zhou
humanWB

Oxid Med Cell Longev

Betulinic Acid Suppresses Breast Cancer Metastasis by Targeting GRP78-Mediated Glycolysis and ER Stress Apoptotic Pathway.

Authors - Yifeng Zheng