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CAMK1 Polyclonal antibody

CAMK1 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 24312-1-AP

Print datasheet

Synonyms

CaM KI, CaM kinase I, CaM kinase I alpha, CAMK1, CAMKI, CaMKI alpha

验证数据展示

  • IHC staining of human colon cancer using 24312-1-AP

    IHC staining of human colon cancer using 24312-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24312-1-AP (CAMK1 antibody) at dilution of 1:200 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with CAMK1 antibody included
  • IHC staining of human colon cancer using 24312-1-AP

    IHC staining of human colon cancer using 24312-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24312-1-AP (CAMK1 antibody) at dilution of 1:200 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with CAMK1 antibody included
  • WB analysis using 24312-1-AP

    WB analysis using 24312-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24312-1-AP (CAMK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 24312-1-AP

    IF Staining of HEK-293 using 24312-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 24312-1-AP (CAMK1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HEK-293 using 24312-1-AP

    IP experiment of HEK-293 using 24312-1-AP

    IP result of anti-CAMK1 (IP:24312-1-AP, 4ug; Detection:24312-1-AP 1:300) with HEK-293 cells lysate 3400ug.

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CAMK1 Ready-To-Use IHC Kit. CAMK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24312-1-AP is CAMK1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen CAMK1 fusion protein Ag19449 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium/calmodulin-dependent protein kinase I
Synonyms CaM KI, CaM kinase I, CaM kinase I alpha, CAMK1, CAMKI, CaMKI alpha
Calculated Molecular Weight 370 aa, 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC106755
Gene Symbol CAMK1
Gene ID (NCBI) 8536
RRIDAB_2879489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14012
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CAMK1 antibody 24312-1-APDownload protocol
IHC protocol for CAMK1 antibody 24312-1-APDownload protocol
IF protocol for CAMK1 antibody 24312-1-APDownload protocol
IP protocol for CAMK1 antibody 24312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biomed Res Int

Effect of Lentivirus-Mediated miR-499a-3p on Human Umbilical Vein Endothelial Cells.

Authors - Huilei Zheng
View Article
mouseIHC

Biochim Biophys Acta Mol Basis Dis

IGF2BP3-stabilized CAMK1 regulates the mitochondrial dynamics of renal tubule to alleviate diabetic nephropathy

Authors - Du Yuan
View Article