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CACNG3 Polyclonal antibody

CACNG3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 13729-1-AP

Print datasheet

Synonyms

Cacng2, CACNG3

验证数据展示

  • WB analysis of HEK-293 using 13729-1-AP

    WB analysis of HEK-293 using 13729-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13729-1-AP (CACNG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13729-1-AP

    IHC staining of mouse brain using 13729-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13729-1-AP (CACNG3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13729-1-AP

    IHC staining of mouse brain using 13729-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13729-1-AP (CACNG3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13729-1-AP targets CACNG3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CACNG3 fusion protein Ag4730 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium channel, voltage-dependent, gamma subunit 3
Synonyms Cacng2, CACNG3
Calculated Molecular Weight 315 aa, 36 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC040005
Gene Symbol CACNG3
Gene ID (NCBI) 10368
RRIDAB_2070085
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60359
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Voltage-dependent calcium channels couple membrane depolarization in a number of cellular processes. These activities are regulated by distinct channels composed of alpha-1, beta, alpha-2/delta, and gamma subunits. CACNG3, one of the eight gamma subunits in human, is a type I transmembrane AMPA receptor regulatory protein (TARP). TARPs regulate both trafficking and channel gating of the AMPA receptors.

实验方案

Product Specific Protocols
WB protocol for CACNG3 antibody 13729-1-APDownload protocol
IHC protocol for CACNG3 antibody 13729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

BMC Cancer

Integrated profiling identifies CACNG3 as a prognostic biomarker for patients with glioma

Authors - Enfang Shan
View Article