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CoraLite® Plus 647-conjugated CA9 Polyclonal antibody
CA9 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 11071-1-AP
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-11071 targets CA9 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC (Intra) |
Tested Reactivity | human, mouse, rat |
Immunogen | CA9 fusion protein Ag1540 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | carbonic anhydrase IX |
Synonyms | CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250 |
Calculated Molecular Weight | 459 aa, 50 kDa |
Observed Molecular Weight | 60-70 kDa |
GenBank Accession Number | BC014950 |
Gene Symbol | CA9 |
Gene ID (NCBI) | 768 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q16790 |
Storage Buffer | PBS with 0.09% sodium azide. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia (PMID:18703501). It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular. Western blotting detected CA9 protein at a molecular weight of 70 kDa (PMID: 31819036).
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 CA9 antibody CL647-11071 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |