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CoraLite®555-conjugated Beta Actin Recombinant antibody

Beta Actin Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, pig, rat, mouse, Canine

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

4H1

Cat No : CL555-81115

Print datasheet

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

验证数据展示

  • IF Staining of MDCK using CL555-81115

    IF Staining of MDCK using CL555-81115

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite® Plus 555 Beta Actin antibody (CL555-81115, Clone: 4H1 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inMDCK cells
For other applications, we recommend the unconjugated version of this antibody, 81115-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-81115 targets Beta Actin in IF applications and shows reactivity with Human, pig, rat, mouse, Canine samples.

Tested Applications IF/ICC
Tested Reactivity Human, pig, rat, mouse, Canine
Immunogen Beta Actin fusion protein Ag14521 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name actin, beta
Synonyms ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin
Calculated Molecular Weight 375 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC002409
Gene Symbol ACTB
Gene ID (NCBI) 60
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP60709
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )

实验方案

Product Specific Protocols
IF protocol for CL555 Beta Actin antibody CL555-81115Download protocol
Standard Protocols
Click here to view our Standard Protocols