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BMI-1 antibody (mAb) (Clone DC9)

Host / Isotype

Mouse / IgG1, k

Reactivity

Human, Mouse

Applications

ICC, IF, WB

CloneNo.

DC9

Cat No : 61089,61090 61089

Synonyms

BMI-1, BMI 1, BMI1, Polycomb, PRC1 complex, silencing, E3 ubiquitin protein ligase, RNF2, RING2, resulting in the ubiquitylation, histone H2A at lysine 119, H2AK119Ub, oncogene, lymphoma, metastatic melanoma, hematopoietic stem cell self renewal, stem cell, sample, clone DC9

验证数据展示

  • BMI-1 antibody (mAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with BMI-1 antibody at a 0.5 ug/ml dilution.

    BMI-1 antibody (mAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with BMI-1 antibody at a 0.5 ug/ml dilution.

产品信息

Tested Applications ICC, IF, WB

Applications Validated by Active Motif: ICC/IF: 0.5 ug/ml dilution For ChIP-Seq analysis of BMI-1, we offer AbFlex BMI-1 Recombinant Antibody (rAb). For details, see Catalog No. 91195.
Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1, k
Class Monoclonal
Type Antibody
Immunogen This BMI-1 antibody was raised against a recombinant protein corresponding to full-length mouse BMI-1.
Full Name BMI-1 antibody (mAb) (Clone DC9)
Synonyms BMI-1, BMI 1, BMI1, Polycomb, PRC1 complex, silencing, E3 ubiquitin protein ligase, RNF2, RING2, resulting in the ubiquitylation, histone H2A at lysine 119, H2AK119Ub, oncogene, lymphoma, metastatic melanoma, hematopoietic stem cell self renewal, stem cell, sample, clone DC9
Molecular weight 40 kDa
GenBank accession numberNP_031578
RRIDAB_2793501
Purification Method Protein G Chromatography
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

BMI-1 is a member of the Polycomb PRC1 complex that is recruited to transcriptionally repressed genes subsequent to histone H3 lysine 27 methylation in order to maintain repression. In the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2, resulting in the ubiquitylation of histone H2A at lysine 119. BMI-1 acts as an oncogene and cooperates with c-myc in the initiation of lymphoma. High levels of expression of BMI-1 are observed in metastatic melanoma, and BMI-1 has been implicated in several other cancers. BMI-1 is required for hematopoietic stem cell self renewal.