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BMI-1 antibody (mAb) (Clone AF27)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

ChIP, ChIP-Seq, IF, IHC, WB

CloneNo.

AF27

Cat No : 39993,39994 39993

Synonyms

BMI-1, BMI 1, BMI1, Polycomb, PRC1 complex, silencing, E3 ubiquitin protein ligase, RNF2, RING2, resulting in the ubiquitylation, histone H2A at lysine 119, H2AK119Ub, oncogene, lymphoma, metastatic melanoma, hematopoietic stem cell self renewal, stem cell, sample

验证数据展示

  • BMI-1 antibody (mAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from mouse embryonic fibroblast (MEF) cells and 4 ug BMI-1 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 10 million sequence tags were mapped to identify BMI-1 binding sites.

    BMI-1 antibody (mAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from mouse embryonic fibroblast (MEF) cells and 4 ug BMI-1 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 10 million sequence tags were mapped to identify BMI-1 binding sites.

  • BMI-1 antibody (mAb) tested by Western blot. 20 ug of K562 cell nuclear extract was run on SDS-PAGE and probed with BMI-1 antibody at 0.5 ug/ml. For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background.

    BMI-1 antibody (mAb) tested by Western blot. 20 ug of K562 cell nuclear extract was run on SDS-PAGE and probed with BMI-1 antibody at 0.5 ug/ml. For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background.

产品信息

Tested Applications ChIP, ChIP-Seq, IF, IHC, WB

Applications Validated by Active Motif: ChIP-Seq: 4 ug per ChIP WB: 0.5 - 2 ug/ml For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background. Individual optimization may be required.
Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen This BMI-1 antibody was raised against a recombinant protein corresponding to full-length mouse BMI-1.
Full Name BMI-1 antibody (mAb) (Clone AF27)
Synonyms BMI-1, BMI 1, BMI1, Polycomb, PRC1 complex, silencing, E3 ubiquitin protein ligase, RNF2, RING2, resulting in the ubiquitylation, histone H2A at lysine 119, H2AK119Ub, oncogene, lymphoma, metastatic melanoma, hematopoietic stem cell self renewal, stem cell, sample
Molecular weight 40 kDa
GenBank accession numberNP_031578
RRIDAB_2615070
Purification Method Protein G Chromatography
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

BMI-1 is a member of the Polycomb PRC1 complex that is recruited to transcriptionally repressed genes subsequent to histone H3 lysine 27 methylation in order to maintain repression. In the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2, resulting in the ubiquitylation of histone H2A at lysine 119. BMI-1 acts as an oncogene and cooperates with c-myc in the initiation of lymphoma. High levels of expression of BMI-1 are observed in metastatic melanoma, and BMI-1 has been implicated in several other cancers. BMI-1 is required for hematopoietic stem cell self renewal.