• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite®594-conjugated p130Cas / BCAR1 Monoclonal antibody

p130Cas / BCAR1 Monoclonal Antibody for FC (Intra), IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra), IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D11C10

Cat No : CL594-67215

Print datasheet

Synonyms

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas

验证数据展示

  • IF Staining of human breast cancer using CL594-67215

    IF Staining of human breast cancer using CL594-67215

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 p130Cas / BCAR1 antibody (CL594-67215, Clone: 2D11C10 ) at dilution of 1:100.

  • IF Staining of human breast cancer using CL594-67215

    IF Staining of human breast cancer using CL594-67215

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 p130Cas / BCAR1 antibody (CL594-67215, Clone: 2D11C10 ) at dilution of 1:100.

  • FC experiment of A431 using CL594-67215

    FC experiment of A431 using CL594-67215

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human p130Cas / BCAR1 (CL594-67215, Clone:2D11C10) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inhuman breast cancer tissue
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 67215-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67215 targets p130Cas / BCAR1 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF-P
Tested Reactivity Human
Immunogen p130Cas / BCAR1 fusion protein Ag10604 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name breast cancer anti-estrogen resistance 1
Synonyms BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas
Calculated Molecular Weight 870 aa, 93 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC062556
Gene Symbol BCAR1
Gene ID (NCBI) 9564
RRIDAB_2920097
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP56945
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 p130Cas / BCAR1 antibody CL594-67215Download protocol
FC protocol for CL594 p130Cas / BCAR1 antibody CL594-67215Download protocol
Standard Protocols
Click here to view our Standard Protocols