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p130Cas / BCAR1 Polyclonal antibody

p130Cas / BCAR1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 16815-1-AP

Print datasheet

Synonyms

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, p130Cas

验证数据展示

  • WB analysis using 16815-1-AP

    WB analysis using 16815-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 16815-1-AP

    IHC staining of human colon cancer using 16815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 16815-1-AP

    IHC staining of human colon cancer using 16815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of RAW 264.7 using 16815-1-AP

    IP experiment of RAW 264.7 using 16815-1-AP

    IP result of anti-p130Cas / BCAR1 (IP:16815-1-AP, 4ug; Detection:16815-1-AP 1:600) with RAW 264.7 cells lysate 4000ug.

  • IF Staining of HeLa using 16815-1-AP

    IF Staining of HeLa using 16815-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using p130Cas / BCAR1 antibody (16815-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of A431 using 16815-1-AP

    FC experiment of A431 using 16815-1-AP

    1X10^6 A431 cells were stained with 0.2 ug Anti-Human p130Cas / BCAR1 (16815-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH.

经过测试的应用

Positive WB detected inHeLa cells, A431 cells, HepG2 cells, T-47D cells
Positive IP detected inRAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16815-1-AP targets p130Cas / BCAR1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen p130Cas / BCAR1 fusion protein Ag10354 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name breast cancer anti-estrogen resistance 1
Synonyms BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, p130Cas
Calculated Molecular Weight 870 aa, 93 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC062556
Gene Symbol BCAR1
Gene ID (NCBI) 9564
RRIDAB_2065460
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56945
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
IHC protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
IF protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
IP protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
FC protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols