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  • Featured Product
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BAG1 Polyclonal antibody

BAG1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 19064-1-AP

Print datasheet

Synonyms

BAG 1, BAG1, BAG1L speicfic, BAG1M, BAG1S, HAP, RAP46

验证数据展示

  • WB analysis of HeLa using 19064-1-AP

    WB analysis of HeLa using 19064-1-AP

    WB result of BAG1 antibody (19064-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAG1 transfected HeLa cells.

  • IHC staining of human testis using 19064-1-AP

    IHC staining of human testis using 19064-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 19064-1-AP

    IHC staining of human testis using 19064-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 19064-1-AP

    IF Staining of HeLa using 19064-1-AP

    Immunofluorescent analysis of HeLa cells, using BAG1 antibody 19064-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • WB analysis of HeLa using 19064-1-AP

    WB analysis of HeLa using 19064-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 19064-1-AP

    IP experiment of HeLa using 19064-1-AP

    IP result of anti-BAG1 (IP:19064-1-AP, 3ug; Detection:19064-1-AP 1:500) with HeLa cells lysate 3440ug.

经过测试的应用

Positive WB detected inHeLa cells, UV treated HeLa cells, Jurkat cells, MCF-7 cells, HEK-293 cells, RAW264.7, HT-1080 cells, HL-60 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

The immunogen of 19064-1-AP is BAG1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen BAG1 fusion protein Ag13555 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BCL2-associated athanogene
Synonyms BAG 1, BAG1, BAG1L speicfic, BAG1M, BAG1S, HAP, RAP46
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 50-55 kDa, 30-33 kDa
GenBank Accession NumberBC014774
Gene Symbol BAG1
Gene ID (NCBI) 573
RRIDAB_10640574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99933
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAG1 have been identified that modulate gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody can recognize all the isoforms of BAG1.

实验方案

Product Specific Protocols
WB protocol for BAG1 antibody 19064-1-APDownload protocol
IHC protocol for BAG1 antibody 19064-1-APDownload protocol
IF protocol for BAG1 antibody 19064-1-APDownload protocol
IP protocol for BAG1 antibody 19064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Oncol Rep

Magnetic gold nanoparticle-mediated small interference RNA silencing Bag-1 gene for colon cancer therapy.

Authors - Wenbai Huang
View Article
humanIHC

Ann Rheum Dis

Unveiling inflammatory and prehypertrophic cell populations as key contributors to knee cartilage degeneration in osteoarthritis using multi-omics data integration

Authors - Yue Fan
View Article