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CoraLite®594-conjugated Alpha Actinin Monoclonal antibody

Alpha Actinin Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E2E1

Cat No : CL594-66895

Print datasheet

Synonyms

actinin, alpha 1, ACTN1, Alpha Actinin, Alpha actinin 1, F actin cross linking protein, FLJ40884, FLJ54432, Non muscle alpha actinin 1

验证数据展示

  • IF Staining of C2C12 using CL594-66895

    IF Staining of C2C12 using CL594-66895

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite®594 Alpha Actinin antibody (CL594-66895, Clone: 1E2E1 ) at dilution of 1:100.

经过测试的应用

Positive IF detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy Alpha Actinin Ready-To-Use IHC Kit. Alpha Actinin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66895-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66895 targets Alpha Actinin in IF applications and shows reactivity with Human, rat, mouse samples.

Tested Applications IF/ICC
Tested Reactivity Human, rat, mouse
Immunogen Alpha Actinin fusion protein Ag28383 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name actinin, alpha 1
Synonyms actinin, alpha 1, ACTN1, Alpha Actinin, Alpha actinin 1, F actin cross linking protein, FLJ40884, FLJ54432, Non muscle alpha actinin 1
Calculated Molecular Weight 103 kDa
Observed Molecular Weight 100-105 kDa
GenBank Accession NumberBC015766
Gene Symbol ACTN1
Gene ID (NCBI) 87
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12814
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

实验方案

Product Specific Protocols
IF protocol for CL594 Alpha Actinin antibody CL594-66895Download protocol
Standard Protocols
Click here to view our Standard Protocols