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AlaRS Monoclonal antibody

AlaRS Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C8H3

Cat No : 67909-1-Ig

Print datasheet

Synonyms

AARS, Alanine tRNA ligase, alanyl tRNA synthetase, AlaRS

验证数据展示

  • WB analysis using 67909-1-Ig

    WB analysis using 67909-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67909-1-Ig (AlaRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • IF Staining of A431 using 67909-1-Ig

    IF Staining of A431 using 67909-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of HeLa using 67909-1-Ig

    WB analysis of HeLa using 67909-1-Ig

    WB result of AlaRS antibody (67909-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AlaRS transfected HeLa cells.

经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells
Positive IF detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67909-1-Ig targets AlaRS in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen AlaRS fusion protein Ag11151 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name alanyl-tRNA synthetase
Synonyms AARS, Alanine tRNA ligase, alanyl tRNA synthetase, AlaRS
Calculated Molecular Weight 968 aa, 107 kDa
Observed Molecular Weight107 kDa
GenBank Accession NumberBC011451
Gene Symbol AARS
Gene ID (NCBI) 16
RRIDAB_2918664
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49588
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).

实验方案

Product Specific Protocols
WB protocol for AlaRS antibody 67909-1-IgDownload protocol
IF protocol for AlaRS antibody 67909-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols