Ago1 antibody (mAb) (Clone 4B8)
Host / Isotype
Rat / IgG2a
Reactivity
Human
Applications
IP, WB
CloneNo.
4B8
Cat No : 61071,61072 61071
Synonyms
验证数据展示
产品信息
| Tested Applications | IP, WB |
| Tested Reactivity | Human |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | This Ago1 antibody was raised against a peptide from the N-terminus of human Argonaute 1 (Ago1) protein. |
| Full Name | Ago1 antibody (mAb) (Clone 4B8) |
| Synonyms | Ago1, Ago 1, argonaute 1, argonaute1, RNAi, siRNA, miRNA, RISC, RNAi Induced Silencing Complex, Argonaute family proteins contain a?PAZ?and a?PIWI?domain, ptsg, paz, piwi, monoclonal, clone 4B8, ago, sample, rna interference, rna processing |
| Molecular weight | 100 kDa |
| GenBank accession number | NP_036331 | RRID | AB_2793495 | Purification Method | Protein G Chromatography |
| Buffer | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
背景介绍
The Argonaute family proteins (Ago1, Ago2, Ago3 and Ago4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage. Ago1 is non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage. Argonaute family proteins contain a PAZ and a PIWI domain.