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AXL Monoclonal antibody

AXL Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3D12F11

Cat No : 68108-1-Ig

Print datasheet

Synonyms

AXL, AXL oncogene, AXL receptor tyrosine kinase, JTK11, UFO

验证数据展示

  • WB analysis using 68108-1-Ig

    WB analysis using 68108-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68108-1-Ig (AXL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • FC experiment of HeLa using 68108-1-Ig

    FC experiment of HeLa using 68108-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human AXL (68108-1-Ig, Clone:3D12F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inNCI-H1299 cells, A549 cells, U2OS cells, A431 cells, HeLa cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68108-1-Ig targets AXL in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, FC, ELISA
Tested Reactivity Human
Immunogen AXL fusion protein Ag14401 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name AXL receptor tyrosine kinase
Synonyms AXL, AXL oncogene, AXL receptor tyrosine kinase, JTK11, UFO
Calculated Molecular Weight 894 aa, 98 kDa
Observed Molecular Weight130-140 kDa
GenBank Accession NumberBC032229
Gene Symbol AXL
Gene ID (NCBI) 558
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP30530
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).

实验方案

Product Specific Protocols
WB protocol for AXL antibody 68108-1-IgDownload protocol
FC protocol for AXL antibody 68108-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols