CoraLite®555-conjugated ATP5A1 Monoclonal antibody
ATP5A1 Monoclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, monkey
Applications
FC (Intra), IF/ICC and More (1)
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
1B10H3
验证数据展示
经过测试的应用
Positive IF detected in | HepG2 cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
FC | See 1 publications below |
产品信息
The immunogen of CL555-66037 is ATP5A1 Fusion Protein expressed in E. coli.
Tested Applications | FC (Intra), IF/ICC |
Cited Applications | FC |
Tested Reactivity | human, mouse, rat, monkey |
Cited Reactivity | mouse |
Immunogen | ATP5A1 fusion protein Ag8119 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle |
Synonyms | ATP5A, ATP5A1, ATP5AL2, ATPM, Cell and organelle markers, hATP1, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, MOM2, OMR, ORM |
Calculated Molecular Weight | 60 kDa |
Observed Molecular Weight | 50 kDa |
GenBank Accession Number | BC064562 |
Gene Symbol | ATP5A1 |
Gene ID (NCBI) | 498 |
RRID | AB_2919670 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 557 nm / 570nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P25705 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL555 ATP5A1 antibody CL555-66037 | Download protocol |
FC protocol for CL555 ATP5A1 antibody CL555-66037 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Immunol Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis |